Are Nanobacteria Making Us Ill?
Amit Asaravala
03.14.05 | 2:00 AM
Olavi Kajander didn't mean to discover the mysterious particles that
have been called the most primitive organisms on Earth and that could
be responsible for a series of painful and sometimes fatal illnesses.
He was simply trying to find out why certain cultures of mammalian
cells in his lab would die no matter how carefully he prepared them.
So the Finnish biochemist and his colleagues slipped some of their old
cultures under an electron microscope one day in 1988 and took a closer
look. That's when they saw the particles. Like bacteria but an
astonishing 100 times smaller, they seemed to be thriving inside the
dying cells.
Believing them to be a possible new form of
life, Kajander named the particles "nanobacteria," published a paper
outlining his findings and spurred one of the biggest controversies in
modern microbiology.
At the heart of the debate is the
question of whether nanobacteria could actually be a new form of life.
To this day, critics argue that a particle just 20 to 200 nanometers in
diameter can't possibly harbor the components necessary to sustain
life. The particles are also incredibly resistant to heat and other
methods that would normally kill bacteria, which makes some scientists
wonder if they might be an unusual form of crystal rather than
organisms.
In 1998, Kajander tried to prove the skeptics
wrong by turning up what he believed to be an example of nanobacteria's
ribosomal RNA, something that only organisms have. But the claim was
squashed two years later by a National Institutes of Health study, which found that the RNA was actually a remnant from a type of bacteria that often contaminates lab equipment.
The debate would have ended there, except for a steadily increasing
number of studies linking nanobacteria to serious health problems,
including kidney stones, aneurysms and ovarian cancer. The studies show
that nanobacteria can infect humans, a find that has helped push
nanobacteria back into the limelight. Now the pressure is on to resolve
the controversy and expose how nanobacteria works -- no matter what it
is.
"It's all pretty exciting stuff," said David McKay, chief
scientist for astrobiology at NASA's Johnson Space Center. "Whether
these are bacteria or not -- it doesn't matter at this point. What
matters is if we can figure out the association between nanobacteria
and kidney stones and develop some kind of countermeasure."
The link between nanobacteria and human diseases was first noticed
by Kajander and microbiologist Neva Çiftçioglu in 1998. The researchers
had observed, through an electron microscope, nanobacteria particles
building shells of calcium phosphate around themselves. They began to
investigate whether such particles played a role in causing kidney
stones, which are also made of calcium compounds. Sure enough, at the
center of several stones was a nanobacteria particle.
Another breakthrough came in 2003 when a team from the University of Vienna Medical Center discovered
nanobacteria in the calcified debris found in tissue samples from
ovarian cancer patients. Meanwhile, several other studies revealed
nanobacteria in samples of calcified arteries.
Sensing a growing need for tools to detect and study nanobacteria, Kajander and Çiftçioglu formed a company called NanoBac
in 1998. The decision was greatly criticized as a conflict of interest
and is still brought up whenever either of the two publishes a new
paper.
Fortunately for the researchers, a 2004 study
by the esteemed Mayo Clinic supported many of their key findings and
helped them regain some of their support. The Mayo study found that
nanobacteria do indeed self-replicate, as Kajander had noticed, and
endorsed the idea that the particles are life forms.
Kajander
and Çiftçioglu were further vindicated this February when patients with
chronic pelvic pain -- thought to be linked to urinary stones and
prostate calcification -- reported "significant improvement" after
using an experimental treatment provided by Nanobac Life Sciences, which now owns NanoBac. The study was conducted by a team at Cleveland Clinic Florida.
There's a lot riding on studies like these. Roughly 177,500 patients
were discharged from U.S. hospitals with kidney stones and related
problems in 2001, according to the NIH. More than 25,000 women in the
United States are diagnosed with ovarian cancer each year. In the same
period, 14,000 Americans die from complications caused by calcified
arteries.
"It brings up a lot of questions," said John
Lieske, who led the 2004 Mayo Clinic study. "How many kidney stones are
caused by this? Are there other calcification-related diseases that are
caused by nanobacteria? Is it infectious?"
Surprisingly, few groups are actually working on answering these
questions. One would be hard-pressed to find more than a half-dozen
research teams around the globe studying nanobacteria full time.
Lieske suggests it's because the field is still relatively young. But
it's clear that there's an additional culprit: the often heated
controversy over whether nanobacteria particles are, in fact, alive.
"There's a reluctance to get into controversial areas. It's hard to get
proposals funded," said McKay. "Most people are waiting until there's a
little more meat on the bones."
Even John Cisar, who led the
2000 NIH study that contradicted Kajander's initial findings, agrees
that the issue has become muddled. Though he maintains his stance that
nanobacteria are not alive, he said in a phone interview that he is not
against further research.
"I'm not saying there's nothing
there," said Cisar. "It's just that we were looking at it from a
microbiologist's perspective. And when we didn't find any signs of
life, we moved on."
Kajander stands by his original assertion
that nanobacteria are life forms. However, he blames himself for
getting researchers hung up on the life question by using the name
"nanobacteria."
"Calcifying self-propagating nanoparticles would have been much better," he wrote in an e-mail to Wired News.
But he added that his regrets about the name don't change the fact that
nanobacteria have "miraculous" properties. Those include a growth cycle
that closely matches typical biological cycles, the ability to form a
shell and the "presence of both mammalian and bacterial components."
It's these properties -- and the potential to save lives -- that keep researchers focused on nanobacteria.
In February, NASA's McKay and Nanobac's Çiftçioglu announced that they had observed
nanobacteria growing at five times its normal rate after they placed it
in an incubator that simulates the microgravity conditions of space.
The findings mean astronauts may be at an elevated risk for kidney
stones on long flights -- something NASA is extremely worried about in
light of its new plans to send humans to Mars.
The findings could also add fuel to nanobacteria research by giving scientists a way to grow cultures faster.
"The trouble with studying nanobacteria is that trying to get enough
material is very hard," said Lieske. "Trying to culture a lot of it
takes time."
Indeed, nanobacteria particles double about once
every three days. In comparison, typical bacteria double about every 20
minutes.
Lieske's group has continued to experiment with
nanobacteria since its 2004 paper. Though he said the team is looking
for evidence of DNA and RNA, he is cautious about saying whether he
thinks the particles are alive or just an unknown form of crystal.
As a possibility, he offered a third option: The particles could be a
form of archaea, a relatively new category of tiny organisms whose DNA
is vastly different from that found in typical bacteria. Over the past
two decades, archaea have surprised
scientists by turning up in places where life was least expected, like
in sulfurous lakes and hydrothermal sea vents.
Whatever the case, the Mayo Clinic team may publish a paper outlining new findings in about six months, according to Lieske.
The world may not be waiting, but a handful of faithful microbiologists certainly will.
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